Polymeric Nanomaterials

The Johns Hopkins Center for Translational Immunoengineering research team is bioengineering nanoimmunomaterial tools (NIMs) with new biomaterials and particles for immunomodulation. These particle-based biotechnologies function through intracellular delivery of nucleic acids, extracellular presentation of biological molecules from biomimetic surfaces, or both. By controlling NIMS physical, chemical, and biological properties, researchers can engineer T cells, dendritic cells, macrophages, and cells in other microenvironments. In many cases, NIMs can target cell-specific delivery. With the aid of NIMs, the efficacy of adoptive cell transfer (ACT) and particle-based acellular immunotherapeutics can be improved in multiple fields and human health applications.

Key properties of PBAEs and their development

Poly(beta-amino ester)s as Gene Delivery Vehicles: Challenges and Opportunities

Synthetic, biodegradable nanoparticles that can genetically reprogram cancer cells and their microenvironment in situ

In situ Genetic Engineering of Tumors for Long-Lasting and Systemic Immunotherapy

A new type of biomimetic particle to induce tolerance

Biomimetic Tolerogenic Artificial Antigen Presenting Cells for Regulatory T Cell Induction

PLGA/PBAE aAPCs as a biocompatible, directly injectable acellular therapy for cancer immunotherapy

Biodegradable Cationic Polymer Blends for Fabrication of Enhanced Artificial Antigen Presenting Cells to Treat Melanoma

Efficient mRNA delivery nanoparticles

High-Throughput Bioassay Enables Tuning of Polyester Nanoparticles for Cellular Uptake, Endosomal Escape, and Systemic Delivery of mRNA

Barcoded nanoparticles

High-Throughput Evaluation of Polymeric Nanoparticles for Tissue-Targeted Gene Expression Using Barcoded Plasmid DNA

siRNA delivery nanoparticles

Photocrosslinked Bioreducible Polymeric Nanoparticles for Enhanced Systemic siRNA Delivery as Cancer Therapy

Gene editing nanoparticles

Poly(Beta-Amino Ester) Nanoparticles Enable Nonviral Delivery of CRISPR-Cas9 Plasmids for Gene Knockout and Gene Deletion

Biomimetic nanoparticles to remove toxins

Biomimetic Anisotropic Polymeric Nanoparticles Coated with Red Blood Cell Membranes for Enhanced Circulation and Toxin Removal

Nanoparticles for cytosolic protein delivery

Carboxylated Branched Poly(beta-amino ester) Nanoparticles Enable Robust Cytosolic Protein Delivery and CRISPR/Cas9 Gene Editing

Nanoparticles to cross the blood brain barrier

Engineered Nanoparticles for Systemic siRNA Delivery to Malignant Brain Tumors

Theranostic nanoparticles

Polymeric Nanoparticles for Dual-Targeted Theranostic Gene Delivery to Hepatocellular Carcinoma